Dr.
Ayodele Oluwayemisi OGUNLADE
Department of Food Technology, The Federal Polytechnic, Ado Ekiti, Ekiti state, Nigeria
Title of the talk: Prevalence and Antibiotic susceptibility pattern of Escherichia coli isolated from raw milk samples from Cow and Sheep milk in Ado-Ekiti, Nigeria
Abstract
The emergence and wide-spread dissemination of antimicrobial resistant bacteria strains is a global phenomenon of great public health and economic implications. There is a rising concern about illnesses resulting from milk consumption due to contamination by E. coli. The aim of this work is to determine the prevalence and Antibiotic susceptibility pattern of E. coli isolated from raw milk samples from cow and sheep in Ado-Ekiti. Isolation and identification of E. coli were done using standard procedures. The PCR was done using 16SrRNA. A total of 186 milk samples (126 from cow and 60 from sheep) were collected from four different locations (Aba Erifun, Aba baba Medinat, Ago Aduloju and Irasa) in Ado-Ekiti. Antimicrobial susceptibility of the isolates was carried out using Kirby-Bauer disk diffusion method. The study revealed that (32/126) 25.39% of cow milk and (9/60) 15 % from sheep milk were contaminated with E. coli. A total of 78 E. coli isolates were gotten. 13 (16.67%) of the isolates were E. coli O157:H7. The Isolate was resistance to amoxicillin-clauvulanic (46.15%), ampicillin (50%), tetracycline (52.56%), ceftazidime (79.48%) augumentin (88.46%), cefotaxime (91.03%). However, they were susceptible to gentamicin (91.02%) ciprofloxacin, ofloxacin, sulfamethoxazole and nitrofurantoin (100%). Screening of resistance genes showed that 6 out of 13 antibiotic-resistant E. coli isolates (46.15%) harbored blaTEM gene, 3 (23.07%) harbored blaCTX-M gene. The study established the presence of resistant genes in E. coli from raw milk samples. Improved hygienic practices by milk handlers during milk handling and pasteurization are recommended to prevent foodborne diseases.
Biography
Dr. Ayodele Oluwayemisi OGUNLADE completed her Ph.D. in 2018 from Federal University of Technology, Akure, Nigeria. She had her M.Sc. in Industrial Microbiology from the prestigious University of Ibadan, Nigeria with a Distinction. She is currently conducting research, teaching and supervising undergraduate students in the department of Food Technology, The Federal Polytechnic, Ado-Ekiti, Nigeria as a Senior lecturer. She has published over 25 papers in high impact journals and has over 12 years working experience in an academic institution. Her current research area is antimicrobial resistance and virulence factors in food borne pathogens and Microbial impact on Food safety, preservation and quality. She has attended both local and international conferences and trainings on Food and Applied Microbiology, Antimicrobial resistance in bacteria pathogens and One Health. She is presently the departmental chairman for In-house training and staff motivation committee and Student Industrial Work Experience Scheme (SIWES) coordinator.
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